| Congresso Brasileiro de Microbiologia 2023 | Resumo: 425-1 | ||||
Resumo:The Brazilian Amazon Forest is recognized for its high biodiversity in fauna and flora. The genus Hevea (H. brasiliensis and H. guianensis) stands out for producing natural latex with socioeconomic importance and, in addition, this genus is considered a natural reservoir of a wide range of microorganisms. Endophytic fungi belonging to the genus Penicillium have been the subject of studies aimed at exploring their biotechnological potential. The objective of this work was to evaluate the isolate 658F5R AM from Penicillium sp. rubber tree endophytic plant as the ability to promote plant growth through the analysis of different negotiations. The ability of isolated Penicillium sp. in promoting plant growth was evaluated in vitro by activities of indole-acetic acid production (IAA), phosphate solubilization (PS), siderophore production (SD) and ammonia production (AP) and specific media were used, qualitatively evaluated regarding the change of colors in the medium or the formation of a halo around the colony. For in vivo analyses, bean seeds were soaked in a suspension of isolated conidia and sterile distilled water (control). After the vegetative stage, the plants were remaining in relation to the number of leaves, shoot and root length, shoot and root dry mass. The isolate of Penicillium sp. showed a positive result for IAA, a common and very important phytohormone for plants, which act mainly in the growth of stems and leaves. For PS, there was formation of a halo around the colony, considering the result as positive, since phosphate solubilizing fungi play a role in the bioavailability of phosphorus in the soil for plants and this can contribute to the reduction of dependence on fertilizers in several crops, for example, for the cultivation of beans. In addition, the isolate showed potential for SD, a secondary metabolite that is important for chelating iron from the environment and providing it to plants, and also for reducing the availability of iron for pathogens. In addition, the Penicillium sp. proven ability to produce AP, one of the forms of direct transfer of evolution, there was no length of roots and shoots of the plants. In vivo, the Penicillium sp. significantly increased shoot and root length by 33.9% and 11.7%, respectively, and shoot and root dry mass by 8.9% and 7.8%, respectively. Therefore, the isolate showed high performance in the different involved in the promotion of plant growth and can be oriented towards its use in agricultural areas. Palavras-chave: Endophytic fungus, Plant growth promotion, Penicillium sp Agência de fomento:CAPES, CNPq e FAPEMIG | |||||